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利用益生菌重組研發豬瘟疫苗
Efficacy testing of Recombinant Probiotics Expressing Subunit Vaccine against Classical Swine Fever Virus
友善列印
作者: 謝政橘、邱淑君、陳瑞祥
中文摘要: 豬瘟是由豬瘟病毒 (classical swine fever virus) 所引起豬隻一種急性、亞急性、慢性、典型及非典型的疾病過程,病毒經口、鼻等黏膜感染是其主要的感染途徑。由於豬瘟的高傳染性和高致死性,嚴重威脅著產業發展,並造成養豬戶巨大的經濟損失。本計畫利用益生菌所具有的免疫佐劑、吸附黏膜、抗膽汁酸等能力,而又具備低免疫抗原性,在動物腸道有一定增殖等特點,作為表達和傳遞病毒抗原之載體 (vector),設計將豬瘟病毒特異性T細胞之抗原決定位E290基因送入益生菌分泌表達型載體,研發益生菌口服型疫苗。本計畫之主要目的,冀望藉由疫苗的研發,降低該疾病所造成之經濟損失,本計畫已完成益生菌載體模式之建立,惟應就抗體之提升再進一步研究。
英文摘要: Classical swine fever virus (CSFV), a member of the genus Pestivirus of the family Flaviviridae, is a small, enveloped, single- stranded RNA virus. Under the natural conditions, pigs are the only susceptible species, and the virus can cause acute, subacute, or chronic disease. Enormous economic losses to pig industries have been caused by CSFV. Therefore, the development of an efficient vaccine against CSFV is of practical significance. Probiotics possess many properties that make them attractive candidates as antigens carriers when applied in conjunction with compounds with pharmaceutical interest, to the mucosae of host animals; in particular, this includes immunomodulators and vaccines. Probiotic organisms are well known for having beneficial effects on the health of humans and animals. In particular, lactobacilli can survive and colonize the intestinal tract and, furthermore, induce nonspecific immune adjuvant effects. The potential of live recombinant probiotics to deliver heterologous antigens to the immune system has been investigated, suggesting the feasibility of using probiotics as a low risk delivery system for oral vaccines. The purpose of the study is to develop a recombinant probiotics carrying CSFV antigens to induce protective immune responses to CSFV infection. We have developed an effective vaccine delivery system for this disease; further studies are in progress aimed at refining and improving our probiotic delivery system for vaccinating swine against CSFV.
備註: 年份:西元 2016 年
期別:第 50 期
詳細內容:
豬瘟是由豬瘟病毒 (classical swine fever virus) 所引起豬隻一種急性、亞急性、慢性、典型及非典型的疾病過程,病毒經口、鼻等黏膜感染是其主要的感染途徑。由於豬瘟的高傳染性和高致死性,嚴重威脅著產業發展,並造成養豬戶巨大的經濟損失。本計畫利用益生菌所具有的免疫佐劑、吸附黏膜、抗膽汁酸等能力,而又具備低免疫抗原性,在動物腸道有一定增殖等特點,作為表達和傳遞病毒抗原之載體 (vector),設計將豬瘟病毒特異性T細胞之抗原決定位E290基因送入益生菌分泌表達型載體,研發益生菌口服型疫苗。本計畫之主要目的,冀望藉由疫苗的研發,降低該疾病所造成之經濟損失,本計畫已完成益生菌載體模式之建立,惟應就抗體之提升再進一步研究。
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