本計畫將純化的口蹄疫O/TW/97病毒連續在倉鼠腎臟細胞株繼代150代，其VP1核酸序列共有10個位置發生變異，在第1至第15代的繼代病毒均未出現變異，從第16代至150代間，繼代病毒的VP1之核酸變異率分佈於0.2至1.3之間

A purified foot-and-mouth disease virus (FMDV) was passaged serially in baby hamster kidney (BHK) cells for 150 passages. Totally 10 and 7 mutations occurred in VP1 nucleotide and derived amino acid sequences of passaged FMDV respectively.