The aim of the present study is to develop a sandwich enzyme-linked immunosorbent assay (sandwich ELISA) which can detect antigen of foot-and-mouth disease virus (FMDV). Anti-FMDV monoclonal and polyclonal antibodies were derived from either recombinant FMDV protein or whole inactivated FMDV. The antibodies were labeled either with horseradish peroxidase or biotin and were applied to the ELISA for antigen detection. Our results indicated that both monoclonal and polyclonal antibodies were able t