豬第二型環狀病毒感染性質體之建立
- 內容
Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS). PCV2 infection is also an important infectious disease in swine and sometimes co-infect pigs with other pathogens. PCV2 was difficultly to be isolated from field samples, and maintained in low virus titer in vitro. In this study, we have constructed the infectious clone of PCV2 to generate stable virus. The infectious clones were transfected into PK-15 cell line and generate new viral parti